The present study conducted industrial analysis, elemental analysis, and energy spectrum analysis on 317 kerogen samples from the shale of the Qingshankou Formation. The samples underwent two calibrations, and the concepts of Ccc, Hcc, Occ, and Ncc were introduced to effectively evaluate the C, H, O, and N content in the kerogen. The results showed that type- I kerogen exhibited higher Hcc and Ncc values, while having lower Occ values. In contrast, type- III kerogen displayed higher Occ values and lower Hcc and Ncc values in the Qingshankou Formation shale in the Gulong sag area. Kerogen II had lower Hcc and Ncc values than type- I kerogen but higher values than type- III kerogen. Additionally, the Occ value of kerogen II was higher compared to that of type- I and type- III kerogen. Ncc primarily exists in the F- F nanocrystals of clay in the form of organic functional groups (NH+3), rather than in the inorganic N (NH4) between clay cells. Trace element analysis indicated that the shale oil reservoir of the Gulong Qingshankou Formation experienced multiple arid and saltine water environments during deposition. The presence of a saltine water environment facilitated the formation of F- F condensation of clay, leading to the adsorption of organic matter with NH+3 functional groups between the F- F nanocracks of the clay sheet. With increasing depth, the rate of increase in the molecular weight of Ncc was observed to be 0. 96 to 2. 04 times higher than that of Ccc. Notably, the Ncc content of kerogen in the lower section of the Qingshankou Formation at depths ranging from 1300 to 2550 m is significantly higher than that in the upper section. This difference can be attributed to the enrichment of algae in the lower section of the Qingshankou Formation and the relatively higher enrichment of Ncc due to the rapid release of Hcc. This indirectly suggests a stronger hydrocarbon generation capacity in the Qingyi area compared to the upper section. The study of Ncc provides valuable insight into the paleoenvironment salinity, clay pore types, and kerogen sources. It has been demonstrated that higher Occ and Ncc values correspond to better shale storage properties and improved oil and gas- bearing potential. Therefore, Occ and Ncc values can serve as indicators to assess the development level of shale oil. The study of Ncc and Occ in the Gulong shale oil reservoir offers significant information on sedimentary environment, geochemistry, clay structure, kerogen organic matter types, diagenetic reservoirs, and reservoir formation. Consequently, these factors warrant attention in future research.