Groundwater is an essential source of water for socioeconomical development and ecosystem maintenance in karst spring catchment in Jinan. The paper analyzed hydrochemistry and isotopic features for the samples from discharge area in the karst spring catchment using tracers such as ions (Cl, NO3, Mg, Ca, HCO3 and SO4), trace elements (Br, Ba and Sr) and sulfur isotope (34S), coupled with hydrological and geological background of this area. Hydrochemical and isotopic analysis reveals different sources of recharge and flow paths during drought and raining periods. There are three series of springs in the catchment. Flow path of Heihu Spring is to discharge directly from the Ordovician limestone, Baotu Spring flow out from the Ordovician limestone and Quaternary sediments, and Wulong Springs, Pearl Spring and #53 well flow through the weak of quaternary sediments and contact zone between limestone and intrusive rock. Groundwater in the spring catchment is recharged from the mixed water of the Ordovician karst water and silicate rock fissure water during raining period, but it is recharged mainly from the Ordovician karst water during dry season. Sulfur of groundwater in this area mainly comes from burning coal, and shows a rising trend.