In order to effectively detect potential geology anomalous bodies in coal bearing formation, such as coal seam thickness variation, small faults, goafs and collapse columns, and provide scientific guidance for safe and efficient mining, the SUMMIT-II EX explosion-proof seismic slot wave instrument, produced by German DMT Company, was used to detect the underground channel wave with the help of transmission method, reflection method and transflective method. Region area detection experiment in mining face had been carried out thanks to the advantage of channel wave, such as its great dispersion, abundant geology information, strong anti-interference ability and long-distance detecting. The experimental results showed that: (1) Coal seam thickness variation in extremely unstable coal seam has been quantitatively interpreted with an accuracy of more than 80% generally; (2) The faults, goafs and collapse columns could be detected and predicted accurately; (3) Experimental detection of gas enrichment areas, stress concentration regions and water inrush risk zone has been collated; (4) A research system of disaster-causing geology anomalous body detection by in-seam seismic survey has been built, valuable and innovative achievements have been got. Series of innovation obtained for the first time in this study indicated that it was more effective to detect disaster-causing potential geology anomalies by in-seam seismic survey than by ground seismic survey. It had significant scientific value and application prospect under complex coal seam conditions.