The Port Island Formation (PIF), a typical Cretaceous red bed in Hong Kong, is dominated by non-fossiliferous, reddish clastic rocks, making it difficult to determine the sedimentary age of PIF precisely. Previous studies assigned the PIF to Late Cretaceous provisionally only on the basis of its stratigraphic sequence and lithology. This study identified a tuffite interlayer in the PIF and a zircon U-Pb age of 128.2±2.7 Ma by LA-ICP-MS method was obtained. It’s the first time to date the depositional age of the PIF with a reliable chronological constraint. With the support of stratigraphic evidence, we concluded that the geological age of PIF should be Early Cretaceous rather than Late Cretaceous. Based on the volcanic history of Hong Kong and Southeast China and the distribution of the PIF in Mirs Bay, it is believed that there was no volcanic activity in Hong Kong in ca. 128 Ma. The tuffite interlayer discovered in PIF was formed by the deposition of volcanic ash, which might originate from remote region outside Hong Kong, in an aquatic environment on Port Island. The identification of the tuffite interlayer, as the response to a volcanic event, has great significance not only to the studies of establishment and regional correlation of the strata system and the geological evolution in Hong Kong, but also to the study of volcanic activities in Southeast China.