The general classification of intermediate-acid intrusive rocks in the metamorphic zone of Gaoligong Mountains as one of the metamorphic terranes of Proterozoic Gaoligong Mountains is problematic regarding the intrusion stage and age, as well as the subsequent metamorphism and deformation. In this study, we investigated granitic gneiss in the metamorphic zone of Gaoligong Mountains based on the 1:50,000 regional geological survey of Qushi Street (2011–2013) and SHRIMP U-Pb zircon geochronology. Results showed that the SHRIMP U-Pb zircon dating of granitic gneiss ranged from 163.5±5.7 Ma to 74.0±2.0 Ma. Thus, the granitic gneiss was grouped into orthometamorphic rocks (metamorphic intrusions). The dating data of granite rocks associated with intense metamorphism and deformation were divided into three groups, 163.5±5.7 to 162.3±3.1 Ma, 132.2–101.0 Ma and 99.4±3.5–74.0±2.0 Ma, which respectively represented three independent geologic events including an important magma intrusion with superimposed metamorphic effects in the late Middle Jurassic, regional dynamic metamorphism and superimposed reformation of fluid action in the early Cretaceous, and dynamic metamorphism dominated by ductile shear and metamorphism starting from the late Cretaceous.