Chitinozoans collected from upper Tremadocian to lower Floian strata of Chenjiahe section, Yichang, western Hubei, China comprise six species belonging respectively to the genera Euconochitina including a new species, Euconochitina fenxiangensis, Lagenochitina and Bursachitina, together with Desmochitina sp. and Eremochitina sp. The chitinozoan succession across the interval is correlated with relevant conodont and chitinozoan biozones and two new regional chitinozoan biozones, the Lagenochitina destombesi Biozone and the Euconochitina symmetrica Biozone are proposed based on their stratigraphic ranges in the Fenxiang to Honghuayuan formations in the Chenjiahe section.