Tetrachloroethene （PCE） is biodegraded by reductive dechlorination with co-metabolism substrates under anaerobic conditions. By inoculating sludge from an anaerobic pool, a biodegradation test of PCE is conducted in the anaerobic condition. In the test, several substrates including methanol, ethanol, formate, acetate, lactate and glucose, are conducive to the conversion from PCE to TCE and 1,1-DCE. The results show the microbe can be cultivated well under the anaerobic circumstances of mixture of sewage （sludge） and soil with the index of COD after eleven days. Degradation of PCE accords with one order reaction kinetics equation. The sequence of the reaction rate constant is Kacetate 〉Kglucose 〉 Klactate 〉 Kethanol 〉 Kformate 〉 Kmethanol, and acetate is an outstanding co-metabolism substratum whose reaction rate constant is 0.6632d^-1.