Based on studies of the reflectance of Ordovician and Silurian zooclasts from 11 sections around the Huangling Anticline, Hubei, China, combined with determination of the conodont colour alteration indices (CAI) and of infrared spectroscopy of dispersed organic matter from the same or adjacent horizons, it is further proved that reflectances of graptolites, chitinozoans and scolecldonts, like vitrinite and natural bitumen, increase with increasing maturation, but follow different reflectance paths. They may be used, therefore, as thermal maturation indicators. Graptolites display optically strong anisotropy. The maximum reflectance measured from graptolite on polished section cut parallel to the bedding plane is the most suitable for determination of maturation of their host rocks. In polished section chitinozoan and scolecodont show reflectance, but no bireflectance owing to their isotropy. The random reflectance of chitinozoans is higher than that of scolecodonts in the case of the same maturity. By analysing the distribution trends of graptolite, chitionzoan and scolecodont reflectances, the Ordovician and Silurian strata can be subdivided into three areas of maturation in the present region. The potential of oil or gas generation is discussed for each area. The result is basically coincident with the distribution pattern of regional organic maturation presented by CAI and infrared spectroscopic determination of organic matter.