Nd, Sr and O isotopic study on the spilite-keratophyre sequence in Xiqiu shows that its ∈_(Nd) values are inthe range of 4.02-5.26, and its ∈_(Sr) values, +1.4-2.6. According to the points of these data in the ∈_(Nd)-T,∈_(Sr)-T and ∈_(Nd)-∈_(Sr) diagrams, the spilite-keratophyre is interpreted as being slightly contaminated by crustalmaterials. Its δ~(18)O values are 3.9-5.0‰. The depletion of ~(18)O in the rocks resulted from the influence ofseawater hydrothermal alteration during or soon after the rock formation. Based on the isotopic characteristicsand available geochemical data, it is believed that the spilite-keratophyre was formed in the well-developedisland-arc environment during the Late Proterozoic subduction of the palaeo-Pacific plate beneath thesoutheastern margin of the Yangtze massif.