Southeast Hubei Province is one of the most important part of the Cu Au Fe Mo metallogenic belt in the middle lower Yangtze River in China, the Jilongshan Au deposit is a large skarn gold deposit in this area. This paper use Re Os isotope dating method for the determination of timing of the Jilongshan gold deposit. The molybdenite Re Os isotopic model age range from 147 7±2 4 to 151 6±4 02Ma with an isochron age of 148 6±1 5Ma, which is alomost similar to zircon SHRIMP U Pb age of 151 6±0 7Ma of granodiorite porphyry in the Jilongshan Au skarn deposit. These data are alos consistent with skarn Cu Fe mineralization age in Southeast Hubei. The Jilongshan skarn Au deposits may be formed in lithosphere extension tectonic setting. Sulfur isotope values are -2 5‰~5 5‰ with an mean value of 2 84‰, eflecting that the characteristics ore forming material originated from magmatic origin. The content of Re in molybdenite is 174 3×10 -6 ~8714×10 -6 , with an average value of 476 2×10 -6 , indicating that the sources of ore forming are deried from a mixture of crust and mantle material.