The Late Pleistocene littoral ridges of southern South America, especially those of the north of Argentinean Patagonia, contain remains of mollusk shells with bioerosion traces. Eleven sites from marine isotope stages (2 from MIS 7, 4 from MIS 5e) and five sites from modern beaches from northern Río Negro Province, with 40 taxa (17 bivalves and 23 gastropods) were analyzed, in the area between west of Baliza San Matías and Las Grutas (41°S). Three ichnogenera were identified in deposits of MIS 7: Entobia, Gastrochaenolites (Domichnia) and Oichnus (Praedichnia) with one ichnospecies (G. torpedo). Six ichnogenera were identified in deposits of MIS 5e: Entobia, Meandropolydora, Pinaceocladichnus, Iramena, Caulostrepsis and Oichnus with one ichnospecies (O. simplex). Seven ichnogenera were identified from modern beaches: Entobia, Meandropolydora, Iramena, Caulostrepsis, Pinaceocladichnus, Gastrochaenolites (Domichnia) and Oichnus (Praedicnia), with two ichnospecies (O. simplex and O. paraboloides). On this basis, it is inferred that Pleistocene benthic communities were constituted from cheilostome bryzoans and polychaete annelids, together with drilling bivalves, predatory gastropods and overall warm-water species of mollusks. Of these, Chama iudicai was found on Plicatula gibbosa from MIS 7 and MIS 5e. The record of warm temperature species suggests a higher sea surface temperature (SST) than the present one and/or the influence of the warm southward Brazilian Current affecting the waters of Golfo San Matías. On the modern beaches surveyed, there is an increase in polychaete annelids, cheilostome bryzoans, temperate-cold species and drilling bivalves, as well as sandy substrate species, which together with an increase of Gastrochaenolites and Caulostrepsis, suggest a colder climate due to the greater influence of the Malvinas Current during the Holocene.