The pollen and spores found in the Early Cretaceous strata of two cores from the Shuangliao Fault Depression were studied systematically, and two palynological assemblages have been recognized. Assemblage I from the Yingcheng Formation is named Paleoconiferus-Pinuspollenites; the assemblage is characterized by an extremely high content of Paleoconifer pollen. Assemblage II from Member 1 of the Denglouku Formation is named Cicatricosisporites-Cyathidites-Pinuspollenites; the assemblage is characterized by the abundant presence of Laevigati spores, but less Paleoconifer pollen. According to the palynological data and zircon U-Pb dating, the geological age of the Yingcheng Formation is thought to be Aptian-Albian, and that of the Denglouku Formation is thought to be Early Albian. On the basis of the composition of each assemblage, we can infer that during the depositional period of the Yingcheng Formation, the paleovegetation was mainly conifer forest, the paleoclimate was consistent with the temperate climate of today, and the paleoenvironment was humid feature. During the depositional period of Member 1 of the Denglouku Formation, the paleovegetation was mainly conifer forest and shrubs, the paleoclimate was consistent with the subtropical climate of today, and the paleoenvironment was humid. The results significantly improve our understanding of the stratigraphy in the Shuangliao Fault Depression, provide biostratigraphic evidence for the division and correlation of Early Cretaceous strata in the Shuangliao Fault Depression, and provide new data for analyzing paleovegetation and paleoclimate in the Songliao Basin.