Marine strata in the Jianghan Plain area are widely distributed with a total depth of more than 8,000 m from the Upper Sinian to the Middle Triassic. Six reservoir caprock units, named Z–?2, ?2–O, S, D–C, P and T1, can be identified with each epoch. The geology, stratigraphy, drilling, oil testing and other basic data as well as the measured inclusion and strontium isotope data in the study area are used in the analysis of the formation and evolution process of marine petroliferous reservoirs in the Jianghan Plain area. This study aims to provide a scientific basis for the further exploration of hydrocarbons in the Jianghan Plain and reduce the risks by analyzing the key factors for hydrocarbon accumulation in the marine strata. Our findings show that in the Lower Palaeozoic hydrocarbon reservoir, oil/gas migration and accumulation chiefly occurred in the early period of the Early Yanshanian, and the hydrocarbon reservoir was destroyed in the middle–late period of the Early Yanshanian. In the Lower Triassic–Carboniferous hydrocarbon reservoir, oil/gas migration and accumulation chiefly occurred in the Early Yanshanian, and the hydrocarbon reservoir suffered destruction from the Late Yanshanian to the Early Himalayanian. The preservation conditions of the marine strata in the Jianghan Plain area have been improved since the Late Himalayanian. However, because all source beds have missed the oil/gas generation fastigium and lost the capacity to generate secondary hydrocarbon, no reaccumulation of hydrocarbons can be detected in the study area’s marine strata. No industrially exploitable oil/gas reservoir has been discovered in the marine strata of Jianghan Plain area since exploration began in 1958. This study confirms that petroliferous reservoirs in the marine strata have been completely destroyed, and that poor preservation conditions are the primary factor leading to unsuccessful hydrocarbon exploration. It is safely concluded that hydrocarbon exploration in the marine strata of the study area is quite risky.