The Jinshan gold deposit is located in the Northeast Jiangxi province, South China, which related to the ductile shear zone. It contains two ore types, i.e. the alteration-type ore and the gold-bearing quartz vein ore. Rb-Sr age dating is applied to both gold-bearing pyrite in the alteration-type ore and fluid inclusion in the gold-bearing quartz vein to make clear the time of the gold mineralization of the Jinshan deposit. Analytical results of this study yielded that the age of the alteration-type ore bodies is about 838±110Ma, with an initial 87Sr/86Sr value of 0.7045±0.0020. However, the age of the gold-bearing quartz vein-type ore is about 379±49Ma, and the initial 87Sr/86Sr is 0.7138±0.0011. Based on the age data from this work and many previous studies, the authors consider that the Jinshan gold deposit is a product of multi-staged mineralization, which may include the Jinninian, Caledonian, Hercynian, and Yanshanian Periods. Among them, the Jinninian Period and the Hercynian Period might be the two most important ore-forming periods for Jinshan deposit. The Jinninian Period is the main stage for the formation of alteration-type ore bodies, while the Hercynian Period is the major time for ore bodies of gold-bearing quartz vein type. The initial values of the 87Sr/86Sr from this study, as well as the previous isotope and trace element studies, indicate that the ore-forming materials mainly derived from the metamorphic wall rocks, and the ore-forming fluids mainly originated from the deep metamorphic water.