In Tunisia, five Bahloul spaced sections, Bargou, Jerisa, Guern Halfaya, Kherij and Gafsa were analyzed for biostratigraphy (foraminifera and radiolarians) and major and trace elements. This high-resolution biostratigraphic and chemostratigraphic integrated analyses for the Late Cenomanian–Early Turonian Bahloul Formation provide new insight into the palaeoceanographic evolution of the southern Tethyan margin. Relative low abundance of related terrigenous Ti/Al and K/Al ratios and enrichment of some productivity proxies such as Ba, Cu, and Ni (organic matter related trace elements) suggests that the Bahloul, deposited during a relatively short period (0.5 Ma), was of relatively elevated primary productivity and minimal detrital input. While higher D* values concurrent with lower Ti/Al ratios are interpreted as caused by enhanced fluvial material contribution, due to more humid climate during the OAE-2. Enhanced humidity triggered probably fluvial influxes, resulted in a sluggish water circulation and consequent anoxic/euxinic conditions favoring the preservation of organic matter at the bottom. Enrichments in redox-sensitive trace metals U, V, and Mo in the Bahloul Formation deposits and redox indices, such as V/(V+Ni), U/Th, V/Cr, and Ni/Co, indicate that oxygen-restricted conditions prevailed during the Late Cenomanian to earliest Turonian times and correlate well with relative abundances of some foraminiferal and radiolarian paleo-environmental relevant indicators. High Baxs values and Uauth may indicate anoxic conditions at least at the water–sediment interface during the Bahloul Formation deposition and provide information about low to moderate sulfate-reduction reactions.