Abstract: Two zosterophyll plants are described from the Lower Devonian (Lochkovian) Xitun Formation of Qujing, Yunnan, China. Xitunia spinitheca gen. et sp. nov. has stalked sporangia laterally attached on the axis in a helical arrangement. Sporangia are dorsoventrally flattened and composed of two unequal valves; the adaxial valve is round in face view, while the abaxial valve is larger than the former, triangular or wedge-shaped, and radially bears long spiny appendages along the distal margin. Xitunia shows new variation of sporangial morphology within the zosterophylls. Zosterophyllum minorstachyum sp. nov. has K-shaped branchings at the basal parts and small-sized terminal spikes, which consist of round to elliptical sporangia arranged helically. This paper provides new data on the diversity of plant types during Lochkovian when rare vascular plants were reported. As for various species of Zosterophyllum in South China, their apparent evolutionary trend of features from the Late Silurian to Early Devonian (Emsian) is discussed.