The giant panda was widely distributed in South China during the Late Pleistocene, with numerous fossil sites, but relatively few intact skeletal materials. We report the newly discovered complete giant panda skull fossil in Yangtianwo Shaft, Huishui, Guizhou, and analyze its burial characteristics combined with cave features.Methods: We have carried out detailed cave survey and cave mapping of Yangtianwao—Baima Cave system. The teeth of giant panda were studied in detail by morphological measurement and compared with those of Ailuropoda melanoleuca baconi and modern giant pandas (Ailuropoda). The fractured bone fragments were dated by AMS—14C dating method.Results: There are favorable conditions for the preservation of fossil in the Yangtianwo Shaft — Baima Cave system because of its special cave environment. According to the measured data, the teeth of Yangtianwo giant panda are slightly larger than those of modern giant pandas, and should belong to the Ailuropoda melanoleuca baconi. The AMS—14 C dating results are 22887~22431 a BP, which is around the last glacial maximum.Conclusions: The discovery of new materials provides rare physical evidence for the distribution range, survival and evolution of giant pandas at the end of the late Pleistocene.