China’s management and service system for cores and samples faces bottlenecks such as unclear processes, poor coordination, and cognitive differences. Concurrently, business management is demonstrating development trends towards institutionalization and refinement, with information technology and multi- parameter digitization technologies becoming key engines driving the upgrade of business management. Based on years of practical experience from the National Repository, this study adopts a vertical flowchart approach. After analyzing key business processes, it attempts to construct an end- to- end business management flowchart encompassing five key operations: “Collection & Acquisition, Arrangement & Preservation, Digitization, Informatization, and Service”. The flowchart developed in this study can clearly illustrate the evolutionary logic and collaborative relationships of each key business. It can provide intuitive operational guidance for repository institutions at all levels, especially newly established ones, and holds significant reference value for establishing a nationally unified, efficient, and collaborative business management system for cores and samples.