New findings have shown that the limestone tablet dissolutional rate in soil increases along with the positive succession of vegetation. The driving force of the tablet dissolution is commonly CO2 and H2O, and their interaction. Soil micorobes strongly impact the CO2 concentration and cycle, and soil carbonic anhydrase (CA) impacts the interaction of the CO2+H2O, however very few studies have been conducted to investigate the changes in soil microbe and CA activity along with the positive succession of vegetation.