The Hongshi gold deposit is located in the Qiugemingtashi—Huangshan ductile shear zone in the east Tianshan Mountains, which mainly controlled mineralization of the gold deposit. The gold mineralization age of the Hongshi gold deposit is 254~259Ma, analyzed by the sericite Ar-Ar dating technique by former researchers. This article aims to evaluate the feasibility of applying Rb-Sr dating technique to constrain the mineralization age of ductile shear zone type gold deposits, taking the Hongshi gold deposit as an example. The Rb-Sr dating is carried out on the fluid inclusions in quartz from the main mineralization stage, yielding an isochron age of 257±4 Ma, the n(87Sr)/n(86Sr) value of 0.70997±0.00016. The isochron age of 257±4 Ma is highly consistent with the gold mineralization age of 254~259 Ma, indicating that Rb-Sr dating on fluid inclusions in quartz is adaptive to date the mineralization age of ductile shear zone type gold deposits. The mineralization age, location relationship and ore forming material source between the Hongshi gold deposit and the Kanggur gold deposit are consistent with each other, indicating that they came from the same mineralization events. The time relationship of mineralization, ductile shearing action, dextral strike slip and uplift cooling proves that the late dextral strike slip and subsequent uplift cooling are the main reasons for the gold mineralization of the Hongshi gold deposit and the Kanggur gold deposit, which located in the same ductile shear zone. Further classification of ductile shear zone type gold deposits, the Hongshi gold deposit belongs to the type of earlier ductile shear deformation, latter brittle ductile deformation and Mineralization superposition.