On the basis of hydrocarbon migration pathways and its time—space effectiveness in the southwestern part of the Gudao oilfield, this paper focused on kinematic and dynamic characteristics of hydrocarbon migration through the synthetical analysis of oil and gas show, fluid property and biomarkers. The research showed that the maturity parameter C29 sterane could effectively prove that hydrocarbon of the 3rd Member of the Shahejie Formation from the Bonan sag and hydrocarbon of the 1st Member of the Shahejie Formation from the Gunan sag are the two sources of this research area, which means the hydrocarbon of the 3rd Member of the Shahejie Formation from the Bonan sag migrated from the Gubei fault, the Guxi fault and other migrate factors, hydrocarbon of the 1st Member of the Shahejie Formation from the Gunan sag migrated from the Gunan fault belt and welldeveloped sands of this formation. While the high formation water salinity and the formation water type could indicate the migration direction and accumulation position of hydrocarbon to some extent. The hydrocarbon show from the buriedhill to Neogene indicated that hydrocarbons accumulated in the buriedhill and Paleogene firstly after its excretion from the source rock, when the oil pools destroyed by faults activities, hydrocarbons migrated again and accumulated in the upper beds, which results the multilayers oil pools and the oil and gas become more and more rich from the bottom to the upper layers. So the research on the direction of hydrocarbon migration and accumulation in the southwest part of the Gudao oilfield has great significance to the research of hydrocarbon accumulation rules at different stages.