Kerogen, adsorbed organic matter, inclusion organic matter and enclosed organic matter in the Lower Palaeozoic source rocks can serve as the source of hydrocarbon regeneration. There are two kinds of hydrocarbon regeneration: one is tectonic movement hydrocarbon regeneration and the other is deep-buried hydrocarbon regeneration. Fluid inclusions formed by these two different hydrocarbon generations are different in type, number, volume, gas to liquid ratio, fluorescent color and intensity. The study of burial history and hydrocarbon generation history of a number of drilling wells and artificial wells shows that Lower Palaeozoic source rocks on the northern slope of the Tazhong area can regenerate hydrocarbons. The area of hydrocarbon regeneration is 9000 km+2, and the time of hydrocarbon regeneration is mainly the Mesozoic-Cenozoic period. Furthermore, the gradual temperature-raising explosion-gas chromatograph method is applied to determine the amount of adsorbed organic matter, inclusion organic matter and enclosed organic matter and their releasing process of highly overmatured carbonate source rocks to evaluate the hydrocarbon regeneration capacity. The study on the hydrocarbon regeneration of the Tazhong area is proved to be very important for deep oil/gas exploration.