Artificial neural network is a frontier science rapidly developed in last decades.This idea is purposed by drawing the results of research from contemporary neural science and artificial neural network is composed of various formal neural units (treatment unit) which are grouped and linked to one another.Three major factors to identify a network system are the characteristics of structure,structure of topological connection and learning regularity.Many people engaging in scientific research of numerous fields pay close attentions to the main charactertics of artificial neural network(complex systems, parallel processing, distributed store, variable structure, show fault, non-linear operation, group coprocessing, self-organization, wide-ranging containment, environment statement and so on) and put forward many kinds of the models of artificial neural network, so the applied systems of multi-subject artificial neural network are formed.Karst environment with multi-medium is a very complicated system that is composed of wate r bodies (precipitation/ surface water/ groundwater), carbonate rocks(differentl y karstificated surface/ under ground structures) and other matters. In addition to the popular characteristics of general environment, karst environment with m ulti-medium itself possesses distinctive characteristics such as multi-layer fe ature, non-linear action and so on, that determine its complicated structure fu nction and properties. Closely connected with air and water bodies,and also cont inually intervened by living things and organisms, karstification leads to the s ensitivity, fragility and vulnerability of the environment.By means of artificia l Neural Network,this paper discusses the organization and structure, internal w ater movement and transmition,time-space distribution and locations of karst sub terranean rivers in karst environment with multi-medium.At the same time, this p aper makes a brave exploration and brings forth new ideas in the orientation of science development,methodology of science, method of recognition and so on