According to the time and space distribution and geochemistry of gold deposits in southwestern Guizhou, in this paper is discussed the possibility of the participation of juvenile fluids in the gold deposit formation. The gold deposits are constrained by the uplift of the Moho, deep faults and mantle-derived magmatic rock bodies, and correspond in space with deromagnetic anomalies, gravity anomalies and circular structures. The study of lead, sulfur, hydrogen and oxygen isotopes and REE geochemistry indicates that the ore material might come from juvenile fluids and crustal rocks. The gold deposits are the product of reworking of crustal rocks by juvenile fluids.