Chromite is a strategically important mineral in short supply in China. Currently, the only chromite deposit under mining operation in China is the Luobusha deposit, located in the eastern segment of the Yarlung Zangbo Suture Zone (YZSZ); however, its production capacity is far from meeting the demands of national economic development. Therefore, exploring chromite deposits with industrial mining value in other domestic regions has become a crucial task that Chinese geologists urgently need to address. This paper reports a new discovery of high-grade massive chromitite in the Cuobuzha ophiolite of the western segment of the YZSZ in Tibet, China. The Cuobuzha ophiolite is situated in the northern subbelt of the western segment of the YZSZ, with the main body of the intrusion being mantle peridotite. This ophiolite is dominated by harzburgite, accompanied by a small amount of dunite. The massive chromitite orebody hosted within it can reach a thickness of 2~3 meters, and the Cr2O3 content of chromian spinel ranges from 56% to 60 wt%, which is typical of high-chromium chromite. Although the Cuobuzha ophiolite and other ophiolite bodies discontinuously exposed in the northern subbelt are all small in surface scale, previous interpretations of aeromagnetic data suggest that the scale of the mantle peridotite basement in the northern subbelt is much larger than that of the extensively exposed mantle peridotite in the southern subbelt. These pieces of evidence indicate that the ophiolite belt in the western segment of the YZSZ has excellent prospecting potential for chromite deposits.