Under the superimposed influence of the Paleo-Pacific tectonic domain to the east and the Mongol-Okhotsk tectonic domain to the north during the Mesozoic, the Daxinganling and its surrounding areas have experienced the Yanshanian intracontinental orogeny and the succeeding large-scale lithospheric extension and thinning event in Eastern China. Conducting in-depth research on the Mesozoic deformation characteristics, ages and genetic mechanisms of the aforementioned region is crucial for elucidating the Mesozoic tectonic regime transformation in East Asia. In this work, a multi-technique approach, including detailed field structural measurement, inversion of paleotectonic stress fields and zircon U-Pb dating, has been performed in the Ganzhuermiao dome in the central-southern Daxinganling. The new results indicate that this dome experienced at least three-phase deformation during the Middle Jurassic-Early Cretaceous: (1) the top-to-the-WSW ductile shearing during the Middle and Late Jurassic (ca. 160 Ma); (2) NNW-SSE contraction during the Late Jurassic to earliest Cretaceous (144-135 Ma) and subsequent (3) NW-SE extension during the middle-late Early Cretaceous (ca. 135-110 Ma). An age-crustal thickness curve, constructed by published the whole-rock (La/Yb)N and Sr/Y ratio data from the late Mesozoic igneous rocks in the central-southern Daxinganling, shows a good correspondence with the above three-phase deformation. It reveals that the regional crust underwent a remarkable thickening from ~41 km to ~66 km during the Late Jurassic to earliest Cretaceous, followed by a rapid thinning to ~45 km at ca. 125 Ma. Combined with the regional tectonic evolution, we propose that the first two stages of deformation represent the effects of the episode A and B of the Yanshanian Movement, respectively. They shared a regional unified contractional setting. The WSW-orientated ductile shearing may reflect an eastward extrusion tectonic model of NE China controlled by the low-angle subduction of the Paleo-Pacific plate beneath East Asia and the closure of the Mongol-Okhosk Ocean. The late NW-SE extension indicates a change of the regional tectonic regime and suggests the peak destruction of the North China Craton, which may result from the roll-back of the Paleo-Pacific plate and post-orogenic collapse of the Mongol-Okhotsk belt.