Sanhetun gold deposit is located in Nenjiang County, Heilongjiang Province. The ore body occurs in the late Triassic granitic mylonite, near the contact zone between the volcanic rocks of the lower Cretaceous Guanghua Formation and the late Triassic granitic mylonite. In order to discuss the source of ore-forming fluid, this work systematically studied the S, Pb, H, O isotopes and inert gas He, Ar isotopic compositions in different minerals collected. Stable isotope results show that: δ34S is between -4.4‰~4.9 ‰，which is similar to meteorite sulfur δ34S value,reflects the contribution of sulfur from deep magma; 206Pb/204Pb of Pyrite =17.284-18~180290，207Pb/204Pb=15.505~15.561,208Pb/204Pb=37.656~38.116; 206Pb/204Pb of Quartz=18.206~ 18.449,207Pb/204Pb=15.526~15.652,208Pb/204Pb=38.050~38.648, which indicates that the ore-forming material mainly comes from mantle; H, O isotopes of Quartz δ DV-SMOW value is - 77.5 ‰~- 96.3 ‰, δ 18OV-SMOW is 7.4 ‰~11.6 ‰, δ18OH2O is - 0.2 ‰~4.8 ‰, which reflects that the ore-forming fluid mainly comes from atmospheric precipitation, and some magmatic water also participates; The 3He/4He ratio of Pyrite fluid inclusions is 1.19～1.54R/Ra, tens to more than hundreds times higher than the 3He/4He ratio of the crust, but obviously lower than the 3He/4He ratio of mantle fluid; 40Ar/36Ar=608.5～922.6, higher than the 40Ar/36Ar composition of crustal fluid (40Ar/36Ar＞295.5);The 40Ar*/4He ratio is 0.14～1.56, with an average value of 0.95. The 40Ar*/4He (0.14) of SHT19 sample data is close to the 40Ar*/4He (0.2) fluid of the crust, and the 40Ar */4He (0.88～1.56) of other samples is much higher than the mantle (0.33～0.56) fluid, indicating that the ore-forming fluid may be mainly meteoric water, but also the crust and deep mantle fluid. According to the regional metallogenic epoch, the Sanhetun gold deposit was formed in the late Early Cretaceous, and the source of ore-forming materials was closely related to the magmatic activity in the Guanghua period of the Early Cretaceous.