Analysis of Archaeal diversity in surface sediment from the Western Pacific
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摘要:
以提取并纯化的西太平洋深海沉积物DNA为模板，利用古菌PCR特异性引物扩增出样品中古菌的16S rDNA片段，构建其克隆文库，建立阳性克隆子RFLP(Restriction Fragment Length Polymor-phism)酶切图谱。据酶切图谱对所获得的90个克隆进行测序，并与数据库中的序列进行比对，从而进行古菌的多样性和系统发育分析。结果表明，沉积物中扩增的16S rDNA古菌序列分别来自泉古生菌(Crenarchaeota)和广古生菌(Euryarchaeota),以Marine Benthic Group B (11.8%)、Marine Benthic Group D (13.6%)、Marine Crenarchaeotic Group (68.69%)为主。少量序列为South African Gold Mine Euryarchaeotic Group (1.07%)、Deep-Sea Hydrothermal Vent Euryarchaeotic Group (1.61%)、UIIB（1.25%）、VALIII（1.79%）、Marine Benthic Group E (0.18%)。以上结果表明西太平洋深海沉积物中有丰富多样的古菌群落。
Abstract:
Diversity of archaeal was studied in deep-sea sediments from the Western Pacific by PCR-RFLP and sequence analysis of 16S rDNA and comparing with the published sequences in GenBank data-base. Archaeal 16S rDNA sequences were within phylums of Crenarchaeota and Euryarchaeota, respectively. The majority of archaeal phylotypes were Marine Benthic Group B (MBGB), Marine Benthic Group D (MBGD) and Marine Crenarchaeotic Group (MCG).Additional sequences grouped with the South African Gold Mine Euryarchaeotic Group (SAGMEG)、Deep-Sea Hydrothermal Vent Euryarchaeotic Group (DHVEG)、UCIIB、VALIII and Marine Benthic Group E (MBGE). These results indicate that archaea are abundant and diversified in surface environment of subseafloor sediments.
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王峰,萨仁高娃,王峰,马学恩.2010.西太平洋深海沉积物古菌多样性分析[J].地质学报,84(8):1105-1111. wangfeng, sarengaowa, wangfeng, maxueen.2010. Analysis of Archaeal diversity in surface sediment from the Western Pacific[J]. Acta Geologica Sinica,84(8):1105-1111.
