Yishu Fault Zone is part of Tanlu Fault Zone in the Shandong Province. It cuts through the Moho surface and separates the central Shandong orogenic belt from the Sulu orogenic belt. The activity of the fault zone determines the tectonic stability of Shandong area. In the past, most of the work focused on the investigation and research of surface or near surface geological elements, and some geophysical exploration work was also carried out in some parts, mostly focusing on sections, and the understanding of the geological structure in the area is relatively limited. In this paper, the regional gravity data of about 14600 square kilometers in the Yishu Fault Zone are collected, and the three- dimensional inversion is carried out. The relative density distribution within 0~30 km underground in the area is obtained. The precise survey sections from Zhuge Town to Wulian gold deposit are comprehensively studied, including gravity, magnetic method and magnetotelluric method. The following results are obtained: ① It is revealed that there is a huge hidden uplift structure underground in the east of the area, with a depth of about 2 km to 10 km, extending from south- west to north- east, and there are three local core areas: about 30 km from Shiqiaozi Town to southwest, between Sangyuan Town, Wulian gold deposit and Xumeng Town, and about 30 km from north to south centered on Hutou Town. ② Faults F1 and F2 observed on the surface are shifted to the right than faults F′1 and F′2 determined by the inverted relative density, and the migration in the north area is greater than that in the south area. ③ The comprehensive profile study of gravity, magnetic method and magnetotelluric sounding shows that the rocks and ores in the north section of Yishu Fault Zone have the characteristics of low relative density, low magnetism and low resistivity, which means that the fault zone has tensile characteristics in the north. To sum up, there are concealed uplift structures in the eastern upper crust of the study area. Changyi—Dadian fault and Anqiu—Juxian fault have tensile fault characteristics in the north and compressive characteristics in the south. Small earthquakes occur frequently in the south of Juxian.