Through the re-investigation and understanding of the “mud crack-like sedimentary structure” in the Qiaotou Formation from late Mesoproterozoic to early Neoproterozoic in the eastern Liaoning, and by comparing with the contemporaneous microbial induced sedimentary structure (MISS) in other areas of the NCC, For the first time, the fractured sedimentary structure discovered in the Qiaotou Formation in Liaoning was identified as MISS.Results: The fissure structure found in the Qiaotou Formation is MISS, which is formed by the physical destruction of microbial mats.Conclusions: The MISS identified in the Qiaotou Formation has spindle-like, three-connected ornithoid and partially connected branched morphology, so it is speculated that the Qiaotou Formation was formed in the sedimentary environment of the upper part of the supratidal zone. This discovery provides basis for stratigraphic and sedimentological studies of the Qiaotou Formation, and is of great significance for studying the evolution of early life on earth and exploring the long-term impact of biota on the atmosphere and hydrosphere.