Taking 6 hot springs in the Chuhe fracture zone of Anhui Province as the main research objects, we analyze the hydrochemical characteristics of the samples of the springs, use hydrogen and oxygen stable isotopes to estimate the elevation of the recharge areas of the springs, and propose the genetic mode of the hot springs. The cation of the hot water are predominated by Ca2+ and Mg2+. Based on the different contents of SO2-4and HCO-3, the water samples can be divided into two groups. The TDS of Group A (SO42- enriched) are lower than those of Group B (SO42- enriched), the hydrochemical types of Group A are of HCO-3—Ca2+·Mg2+ type, the hydrochemical types of Group B are of SO2-4—Ca2+·Mg2+ type (but the hydrochemical type of AH16- 4 is of SO2-4—Ca2+ type). The total content of rare earth element content of the Group A is higher than that of the Group B, the normalized patterns of Group A and Group B are relatively flat, and they all show high REEs depletion after NASC normalization and exist Eu positive abnormality. The hydrogen and oxygen stable isotopes of the water samples indicate that the hot springs are of meteoric origin, the temperature of the recharge areas is approximately 13~15℃. The elevations of the recharge areas of the hot springs of Group A are 120~160 m, which is lower than that of Group B (200~260 m). The estimated temperatures of the geothermal reservoirs of Group A are 45~70℃, which is lower than those of Group B (70~105℃). Groundwater undergoes a deep circulation and obtains heat from heat flow, and rises up to the land surface.