REE compositions in the Gaoyou Depression, North Jiangsu Basin, eastern China, are compared to the REE fingerprints of their possible sources. The REE patterns show that source rocks were mainly derived from the Proterozoic granite—gneiss from a lowgrade metamorphic basement of the Sulu—Dabie orogen belt and the Zhangbaling Uplift to the west. It is inferred that the source rocks in studied area are Itype high potassium granitic gneiss. Whereas, the Neoproterozoic spilite—quartzkeratophyre and the Mesozoic igneous rocks widely distributed in the Zhangbaling Uplift, the eclogite distributed in the southern Dabie Mountains and the Mesozoic intermediate—acid intrusive rocks widely outcropped in Ning—Zhen（Nanjing—Zhenjiang） Mountains have negligible effect on provenance of the Gayou Depression. Source direction analysis discovered that the ratios of light REE (LREE) to heavy REE (HREE) \[(La/Yb)N\] are coincident well with the mineralogical maturity index (MMI). The (La/Yb)N values can correspond well with the sum of REEs (∑REE), and the Eu anormaly values (δEu) can be used in exact judgement of source direction. In period of E2d1, source rocks in the Gaoyou Depression mainly come from the next four orientations: the northwestern Zheduo Salient, the eastern Wubao Salient, the southern Tongyang Uplift and the southwestern Lingtangqiao Salient. Correlative analysis between sedimentary subfacies and REE parameters reveals that, fractional degree of REE \[(Gd/Yb)N\] changed regularly with sedimentary facies. The average (Gd/Yb)N values increase with the maturity indexes of detrital rocks decreasing from delta to fan delta and to nearshore subaqueous fan. This trend is also revealed in different sedimentary subfacies of the same sedimentary facies. Hence, REE parameters especially the (Gd/Yb)N values can well respond to the types of sedimentary subfacies.