Chemical behavior of chalcophile elements in the process of magma evolution and sulfide segregation is a window to demonstrate the formation of sulfide deposits. One direction of development of the study of sulfide deposit is to trace the behavior of chalcophile elements through laboratory results. The present paper summarizes the behavior of sulfur and chalcophile elements in magma evolution, and demonstrates their application in the study of magmatic sulfide deposits. They are discussed from five factors: ① by the study of sulfur solubility in basaltic magma, four factors control the sulfide segregation: magma mixing, rapid temperature decrease, crustal contamination, and rapid crystal fractionation; ② according to the distribution of Ni between olivine and silicate magma, we simulate the correlated change of Ni in olivine to Fo;③ the D values of Ni—Cu and PGE between sulfide liquid and silicate melt is are summarized, and the controlling factors to D value and R factor are discussed also; ④ when olivine is surrounded by sulfide liquid, the interaction will happen, and Ni content in sulfide liquid can be estimated; ⑤ by the distribution of chalcophile elements between MSS and sulfide liquid, the facts of oreforming zonations are summarized. At the end, the present problems and direction of development are analyzed and suggested.