Giant pandas were widely distributed in South China during the Late Pleistocene, with numerous fossils, but relatively few complete bone materials. This article reports the latest discovery of a complete skull fossil of a giant panda at the Yangtianwo shaft in Huishui, Guizhou. The special cave conditions provide favorable conditions for the preservation of fossils. According to the measured data, the teeth of Yangtianwo giant panda are slightly larger than those of the existing giant panda, and should belong to the Ailuropoda melanoleuca baconi. The AMS-14C dating results are 22887-22431 a BP, which is around the last glacial maximum. The discovery of the new material provides a rare empirical evidence to infer the distribution range, survival and evolution of giant pandas at the end of the late Pleistocene.