In order to explore the geothermal geological conditions in the Ganyu—He’an Lake area of the Subei Basin(Northern Jiangsu Basin), Jiangsu Province, on the basis of understanding the geological and geophysical conditions of the area, the methods of geothermal geological survey, Controlled Source Audio Magneto Telluric method （CSAMT）, radon gas measurement, and geothermal measurement were used for detection. The position and distribution characteristics of faults within a depth of about 2500 m are comprehensively analyzed and studied by geophysical prospecting. The research results show that the distribution of geothermal resources in the survey area is closely related to the fault structure, and has basic thermal storage hydrogeological conditions such as “heat source, fluid source, channel, thermal storage, caprock” for the formation of geothermal, and has a good prospect area for exploration. The NWW- trending faults in the area are favorable locations for geothermal resource exploration. This study adds a new geological and geophysical basis for the geothermal genesis and geological structure of northern Jiangsu, and has important guiding significance for the rational development and utilization of geothermal resources.