Paleolatitude evolution could provide a general paleo location framework for explaining the paleoclimate change and tectonic deformation in geological time. Strengthening the paleolatitude study of the Hoh Xil Basin in the north—central Tibetan Plateau is important for understanding the history and mechanism of the tectonic uplift process in the north—central Tibetan Plateau, elucidating the coupling relationship between the origin and evolution of the East Asian monsoon and the uplift and deformation of the plateau, and exploring the dynamics of the arid climate indicated by the Late Eocene sporulation in the Hoh Xil Basin. In this paper, we choose a sub- basin of the Hoh Xil Basin, Tuotuohe Basin (TTHC section) as the target for paleomagnetic research and reconstructing its paleolatitude evolution history during the Cenozoic. Methods: Samples from the TTHC section are got every 1 meter. Typical samples are selected for rock magnetic experiments, and carriying out stepwise thermal demagnetization experiments on the paleomagnetic specimens collected in TTHC section. The inclination of data is corrected by the E—I test. Results: Rock magnetism indicates that the carrier minerals are magnetite and hematite, while hematite is the main carrier mineral. The paleomagnetic result indicates that there is an evident shallowness in inclination in TTHC section when comparing with the expected inclination of the Eurasian pole at ~35 Ma and the volcanic inclination in Wulanwula Lake, which locates at the western of our section. After E/I adjustment, the paleomagnetic direction at ~35 Ma in TTHC section is Dec=358. 5°, Inc=44. 4°, a95=5. 7° and the paleolatitude is 26. 1°N. Conclusions: After synthesizing the paleolatitude results from Gongjue Basin, Gaize Basin, Nima Basin, Nangqiang Basin, Xialaxiu Basin and Wulanwula Lake and this study, we get the paleolatitude evolution history of the Tuotuohe Basin during the Cenozoic (the reference point is TTHC section). Paleolatitude results indicate that the Tuotuohe Basin located at 26. 1° at 35 Ma and the climate is controlled by the sub- tropical high pressure belt, which could explain the arid climate suggested by the pollen and lithology. At 24 Ma, Tuotuohe Basin gets to present latitude and the surface crustal shortening ceased or reduced.