Objectives: Study on subsoil interface processes impact on dissolution rate is helpful for the evaluation of the dissolution rate changes in different land uses and its controlling factors, but also for determination of regional or watershed scale intensity of karst processes and carbon cycle under a given climatic condition. Methods: Four representative land uses were selected for 2- year long standard tablet (tablet is round with a diameter of 40 mm and a thickness of 3 mm) test in Shilin Geopark, Yunnan, one of UNESCO natural heritage sites of Southern China Karst. Tablets were placed at subsoil interfaces and different depth of soil profiles in preliminary forest, artificial pine land, grass land and rock desertification land. Meanwhile, the measurement of soil physical—chemical properties, monitoring of soil moisture with 1- hour interval, seasonal soil CO2 contents measurement were deployed.Results:The results showed that the mean subsoil dissolution rate in different land uses is 82. 3 mg/a, with maximum value of 285. 7 mg/a at 50 cm soil depth of preliminary forest and minimum value of 17. 4 mg/a at 10 cm soil depth of rock desertification land. In general, subsoil annual mean tablet weight loss in descendingorder is primary forest (182. 6 mg), grass land (76. 4 mg), artificial pine (46. 5 mg) and deserted land (23. 8 mg). Subsoil dissolution quantity at soil—rock interface ranges 17. 7~68. 8 mg/a with a mean value of 44. 7 mg/a, and the value of different landuses in rising order is pine land(17. 7 mg/a), deserted land(47. 6 mg/a) and grass land(68. 8 mg/a). subsoil mean dissolution quantity at soil—root interface is 43 mg/a. Dissolution rate in soil is greater than that on rock surface, the rate in primary forest is much larger than that in other three land uses. Conclusions: Subsoil dissolution rate in grass land and primary forest goes up with soil depth increase, especially the latter with a remarkable growth, implying positive evolution of vegetation could improve karst processes exceedingly thus accelerate carbonate rock’s dissolution rate. It is estimated that dissolution rate in the prime of formation of Shilin(stone forest) ranges 35. 1~46. 8 mm/ka, using the rate data in preliminary forest.