Junggar basin is one of the most important basins producing multi- energy mineral, and Toutunhe area in the southern Junggar basin is the prospect section of the sandstone- type uranium ore prospecting. In this paper, based on the field investigation, LA- ICP- MS U- Pb dating and Hf isotope analysis of detrital zircons from the ore- bearing target layer of the sandstone- type uranium deposit in the southern Junggar basin have been carried out and the zircon U- Pb age spectrum, Hf isotope and model age were established. By comparing with the age of magmatic activity and combining with the paleocurrent data, we further analysis the provenance of the strata and the uranium source in sandstone- type uranium deposits. Four sandstone samples from the Jurassic and the Lower Cretaceous were collected for detrital zircon U- Pb dating. The analysis result shows that the detrital zircon U- Pb ages were divided into four peak age groups: 140~180 Ma, 240 ~ 320 Ma, 340~420 Ma, 440~480 Ma, and the age is evenly distributed within the 210~480 Ma range; The provenance of the Lower Jurassic strata with sandstone- type uranium mineralization was derived from the north Tianshan area; The materials from central—south Tianshan area began to increase and dominate in Middle Jurassic strata. After that, the provenance began to change to the north Tianshan again since the late Middle Jurassic. The provenance of Early Cretaceous strata was mostly from the North Tianshan, and a small amount from the Bogda Mountains. The uranium source of sandstone- type uranium deposits in the southern margin of the Junggar basin should be related to the Paleozoic acidic magmatism in Tianshan area, and its mineralization should have occurred in a relatively stable environment.