Objectives: Stable carbon isotope is a useful and powerful tool for tracing the cycle process of dissloved inorganic carbon(DIC) in karst dynamics system and studying the formation and evolution of geohydrochemistry of groundwater in a watershed. Methods: For revealing the evolution and control factors of karst groundwater in Liulin spring area, the main ion components and carbon isotopes of dissloved inorganic carbon (δ13 CDIC,VPDB) in 29 karst groundwater from spring supply area, runoff area, discharge area and deep buried area, were sampled and analyzed. Results: The results showed that the δ13 CDIC,VPDB of karst groundwater in the study area has a large variation range, with a maximum value of -8.19‰, a minimum value of -13.35‰, and the mean value of -10.09‰. The δ13 CDIC,VPDB values from the recharge area, to the runoff area, to the discharge area and then to the deep buried area show a constant weight gain. The proportion of CO2 in DIC from soil is the highest in recharge area, ranging from 46.22 % to 58.04 %, with a mean value of 51.13 %, followed by runoff area, ranging from 36.22 % to 58.37 %, with a mean value of 42.05 %, and the lowest proportion in discharge area and deep burial area, ranging from 37.61 % to 41.52 % and 35.61 % to 42.26 %, with a mean value of 39.38 % and 38.28 %, respectively. Conclusions: Thus, from the recharge area to the runoff area, to the discharge area and then to the deep burial area, the proportion of CO2 in DIC from soil decreases and the proportion of carbon from carbonate rocks increases due to the increase of runoff path and sulfuric acid participation in carbonate dissolution.