Objectives: Jiling syenites occur in the Jiling batholith, developing metasomatiterelated uranium deposit which is rarely presented in China. To explore, the genesis and tectonic significant, petrographic, geochronologic, and geochemical analyses were conducted on the Jiling syenites.Methods:Field investigation, syenite sampling and observation under a microscope were performed to obtain lithology, LAICPMS zircon UPb dating, analysis of geochemical characteristics of major, trace and rear earth elements, isotopic geochemical analysis were used.Result: Both plagioclase and quartz grains as ellipse or round-shape in the K-feldspar, acicular apatite and porous amphibole indicate that the Jiling syenites may be formed by magma mixing. LAICPMS zircon UPb dating reveals the Jiling syenites emplaced at 427±3.6Ma, which is consistent with those of collisionrelated granitoids in northern Qilian Mountains area. The Jiling syenites show intermediate SiO2 contents, total alkaline contents ranging from 8.49% to 11.74% with K2O/Na2O from 1.07 to 1.20, can be classified as shoshonitic type. In the Jiling syenites, Rb, Ba, Th, U and Pb are enriched, while Nb, Ta, P and Ti are depleted; REE contents are high with moderate negative Eu anomalies; the initial Sr isotopic compositions display ranging from 0.70798 to 070799, and εNd(t) from -3.57 to -3.35, which fall in the Itype granitoids area of northern Qilian Mountains area. Combined with petrographic, geochronologic, and geochemical characteristics, writers consider that the Jiling syenites were formed by mixing of crustalderived and mantlederived magmas.Conclusions:The formation of the Jiling syenites may have relationship with collision between the Alax and Qilian—Qaidam Blocks. Due to the closure of the PaleoQilian Ocean at 445 Ma, the Qilian—Qaidam Block collided with the Alax Block, and subducted underneath the Alax Block. It would lead rightlateral strikeslip of the Mountain Longshou fault, and generate in an extensional environment. The continental crust heated by the upwelling of asthenosphere, and generate the felsic magmas. Mixing of the felsic magma and the mantlederived magma formed the Jiling syenites.