Nowadays in China, the oil and gas development of lacustrine dolostone has been industrialized in the Hetaoyuan formation of Paleogene in Biyang depression, the Xiagou formation of Cretacic in Jiuxi depression and the Fengcheng formation of Palaeozoic-Mesozoic Permian in Junsggar basin, which indicates a good evaluation prospect of the domestic likewise reservoirs. New oil and gas exploration has been discovered in the ES35 lacustrine argillaceous dolostone reservoir of Tanggu Oilfield. The reservoir is interactively formed by the lamina of the argillaceous dolostone and dolomitic mudstone with unequal thickness, which is proved to be weak reduction semi-deep water lacustrine deposition by laboratory assay analysis. It has the feature of double porosity media of fracture and matrix, with the micro-mesoporous rate above 50%. It is a new nano-scale unconventional carbonate reservoir. Based on the core observation and oil/production test data of this kind of reservoir, the oil and gas content affecting factors of lacustrine argillaceous dolostone are analyzed by the thin section analysis, the X-ray diffraction analysis, the SEM analysis, the physical properties analysis, the mercury intrusion analysis and the nitrogen adsorption test at low temperature. The research results show that the ES35 oil-bearing influence factors is closely related to the diagenetic mineral components and development, the reservoir space and type, the micro-fractures development, the distribution of abnormal overpressure space, etc. The proposed evaluation analysis method has great significance for the unconventional oil exploration.