Guizhou Qinglong Antimony Ore is an important antimony deposit in China. Previous studies found that many of hydrocarbon inclusions in Qinglong Antimony Ore Field. But the understanding of the hydrocarbon source is not clear. The discovery of paleo oil reservoir in Qinglong Antimony Ore Field makes sources of hydrocarbons with a new possibility, and show new oil and gas exploration area for Southwest Guizhou Depression and Emeishan Basalts. Through fluid inclusions study, we discuss the evolution of paleo oil reservoir.Methods:We chose calcite veins and bitumen in calcite veins mill made inclusions sample, to do the microscopic observation and Raman spectroscopy detect, and measuring the uniformly temperature and freezing point temperatures of salt water inclusions. By analyzing the characteristics of Raman spectroscopy to determine characteristics of fluid which captured when inclusions formed. Use uniformly temperature and capture temperature calibration curve calculated the capture temperature. According to the relationship between the burial depth and temperature relationship, calculate the depth of paleo oil reservoir formation. Combined with the antimony mineralization time, determine the relationship between paleo oil reservoirs and antimony ore.Results:By analyzing the Raman Spectroscopy characteristics of bitumen, this paleo oil reservoir has a high degree of evolution. The inclusions captured liquid petroleum when it formation, and converted into natural gas and bitumen in the geological process. The main component of natural gas is methane. A large amount of natural gas with methane is generated in the process of evolution. Presumably there exists ancient gas reservoir. The capture temperature of inclusions about 144~177 ℃, corresponding to the capture depth about 3169~4185 m. Speculated that the formation of the paleo oil reservoir earlier than antimony ore.Conclusions:The well preserved area in Southwest Guizhou Depression has the potential of natural gas exploration. A wide range of hydrocarbon migration occurred prior to the formation of antimony ore. Ancient reservoirs and antimony ore in the formation context of time may indicate the existence of a relationship between them.