The Shazhou uranium deposit in Xiangshan orefield, Jiangxi Province,is located in the south-west of Gan-Hang uranium metallogenic belt in Southern China, in the west of Southern China vocalnic belt of terrestrial facies. We have systematically collected pyrites and calcite formed at mineralization epoch, then took isotopic tests. The results show that the δ13CV-PDB andδ18OV-SMOW of calcites is -3.2‰~-7.4‰，8.5‰~15.2‰, and it in ore-forming fluids was mainly derived from the Mantle. the n(40Ar)/n(36Ar) of fluid inclusions in pyrites is 303~326, the n(3He)/n(4He) is 0.193~2.946 Ra, which is resulted from that Crust-derived fluids mixed with Mantle-derived fluids at different ratios.