The Tonglushan Cu—Fe deposit, located in the southeastern Hubei Province, is a large skarn deposit. Its wallrock consists of the Triassic marble and dolomitic marble. This caused the development of calcium and magnesium complex skarn mineral assemblages, including garnet, pyroxene, hornblende, epidote, phlogopite et al. This paper describes the characteristics and occurrences of minerals from each mineralization stage, then analyses their compositions using electron microprobe. Garnets formed in three stages and vary from grossular to andradite from early to late in composition. Garnet and epidote of oscillatory zoning imply the concentration of Fe increased gradually during the evolution. Pyroxenes belong to diopside. Amphiboles consist of tremolite, pargasite and slight actinolite. The ratio of Mn/Fe of garnet and pyroxene may be related to mineral metals. The deposit has the positive and relatively narrow δ34SV-CDT values, between 0.6 ‰ ~ 3.8 ‰.δ13CV-PDB values of calcites from quartz—sulfide stage vary from -2.9 ‰ ~ 6.3 ‰, δ18OSMOW values change between 9.6 ‰ and 12.6 ‰, but these two values in postore calcites are obviously increased, with -0.9‰ ~ 1.3 ‰ of δ13CV-PDB value and 15.2‰ ~ 17.3 ‰ of δ18OSMOW values, tend to the values of wall rock. The studies indicated that sulfur and carbon in mineralization stage derived from magmatic source, but carbon in carbonate stage possibly produced exchange interaction with the strata.