Microbial prospecting technology for oil and gas (MPOG) is characterized by low cost, simplicity,convenience, and low ambiguity. It can be based on anomaly detection of microorganisms,can be evaluated and forecast oil and gas underlying the existence and nature of possession. Thus it is possibly suitable for this field. This is based on abnormity of microorganisms, combined with regional geological data, drilling and logging, etc, the authors having carried out an analysis of microorganisms in an anomalous region of the Shengping oil field of Daqing area, and delineation of an obvious anomalous zone. Through the relationship between the microbial abnormity zone and the adjacent areas of anomalous was compared with that of the known developed zone in the Shengping oilfield, a preliminary study the hydrocarbon potential of the Shengping oilfield was conducted. At last, in the main premise of microbial abnormalities, combination of fault area, geological features, as well as the deployment of the studied region, two evaluationcontrolling wells were planned and drilled in the studied area. The MPOG results were verified to be consistent between the hydrocarbonbearing conditions of the drilled 2 wells and MPOG. According to the results of microbial exploration Shengping oilfield added to new proved oil reserves, which reveals the huge potential of application of the new technique of MPOG.