The avian perching behavior is crucial for birds. There are close evolutionary relationship in adaptation between the shape and structure of avian hind limb bones. In addition, the analysis and deduction for perching behavior in Mesozoic birds is a hot research field of fossil birds. In this paper, by contrasting the morphological variations and statistic analysis of the hind limb bones in abundant living birds, we focus on the length variation of hindlimbs, the characteristic diversity of metatarsal trochlea in distal tarsometatarsus, the phalangeal length variation in pedal middle digit and the claw curvature of middle digital claw and compare them with corresponded perching behavior. Then, we apply discriminative characteristic and valid variable to infer Mesozoic birds' perching behavior. By systematically analyzing the perching behavior of Mesozoic birds, We reach the conclusion that large numbers of basal birds with perching behavior maily belong to climbing group, and only minority species belong to arboreal life group. The perching behavior of enantiornithines is close to that of arboreal birds. The perching behavior of ornithurines could be arboreal or terrestrial.