Seventeen new 40Ar/39Ar analyses reported for ten Tarim Precambrian basement samples from the Kuluketage area, are applied to reconstruct the regional thermo-tectonic history together with previously published data. Eight samples were taken adjacent to the Xingdi and Xinger faults, major structures in the study area, whereas a further two were sampled at some distance from the faults. 40Ar/39Ar data from the latter record rapid cooling following a Neoproterozoic magmatic/metamorphic event and mild Paleozoic thermal disturbance. Paleozoic 40Ar/39Ar ages from the study area, as well as from the Central Tianshan and eastern Southern Tianshan suggest two strong deformational periods at ~390 Ma and ~300 Ma. During the older period, argon isotopic systems were reset/disturbed by high temperature related to arc magmatism resulting from subduction of the South Tianshan paleo-oceanic crust, possibly in combination with reactivation of Precambrian faults. The younger period is characterized by widespread late Carboniferous-early Permian intracontinental deformation, which is related to the final amalgamation of the Central Asian Orogenic Belt. Previously published apatite fission track data attest to a long history of post-collisional cooling, which is attributed to continued propagation of deformation within the Central Asian Orogenic Belt.