SHRIMP U-Pb dating was performed on the dunite and harzburgite samples collected from the Kangjinla No.11 chromite in the Luobusha ophiolite, Tibet. Age results of the two samples are not coupled. Dunite sample has an age range from from 125.6±2.6 Ma to 1791±23 Ma (from Archean to Early Cretaceous), with a minimum weighted average value of 130.0±2.8 Ma (2.1%) for four zircon samples. Harzburgite has an age range from 208.9±3.7 Ma to 2770±44 Ma (from Archean to Late Triassic) with a weighted average value of 209.7±5.2 Ma (2.5%) for two zircon samples. The complex characteristics of zircon grains imply the multi-stage geological evolution. Laser Raman Spectroscopy analysis shows that zircons in dunite and harzburgite contain inclusions of crustal material consisting of apatite, feldspar and quartz, indicating its crustal genesis and recording a process of crustal contamination of the mantle. In combination with a large amount of unusual mantle minerals discovered in the Kangjinla chromite and mantle peridotite, there might be a cycle process that crustal material returned surface from oceanic ridge after subducting to mantle, which also proves a multi-stage formation model of the podiform chromitite.