Abnormal high pressure helium-rich natural gas was first discovered in the Triassic Zhouchongcun Formation in Well WWY1 of the Wuwei basin. Gas composition analysis of two gas samples showed that the volume proportions of He were 4.51% and 4.56%, respectively, which were much higher than the industrial utilization standard (0.1%). The two values of 3He/4He are 5.50×10^-8 and 6.40×10^-8 respectively, and the proportion of mantle-derived helium is only 0.32% and 0.40%, which typically suggests crust-derived helium. The integrated analysis of δ13CCH4, δ13CCO2, CO2 content and N2 content indicates the gas has an organic origin, which is associated with the widespread development of Permian hydrocarbon rock in the study area. Analysis of regional geological and deep geophysical data suggests that the helium source rocks are most likely derived from Paleo- and Meso-Proterozoic basement granites. As an important channel connecting deep crust and shallow strata, the Yangtze River deep fault zone plays a key role in helium migration. When the helium-rich fluid migrates to the shallows and meets the carrier gas reservoir of natural gas, the helium concentration of the fluid decreases rapidly and a large amount of helium diffuses into the carrier gas reservoir to form the helium-rich gas reservoir. During this process, active fault systems and fractures served as effective migration channels. The Triassic Zhouchongcun Formation in the sag has formed an ultra-high pressure confining condition under certain burial depth conditions, which has prevented the upward diffusion of helium-rich natural gas. The fractured dolomite reservoir under the gypsum rock layer provides a storage space, and has a good spatial and temporal configuration of the storage cap. There are several similar basins along the Yangtze River (e.g.Wangjiang basin), indicating that there is a very favorable potential for helium exploration along the Yangtze River Area. In addition, the helium accumulation model proposed in this study can also provide important guidance for regional helium exploration.