Ambers are fossils formed from resinous polymers produced by the metabolism of ancient plants. The organic matter in amber is preserved in a stable and closed environment and could provide a good record of information about the ancient plants and palaeoenvironment during the formation of ambers. In this study, the organic geochemical composition, stable isotope and carbon isotope of individual compounds in ambers from the Shenbei coalfield were investigated using gas chromatography- mass spectrometry (GC- MS) and elemental analysis isotope ratio mass spectrometry (EA- IRMS). The results show that the saturated hydrocarbons in amber are dominated by diterpane compounds (73. 45%) and the aromatic hydrocarbons are dominated by abietane- type compounds (56. 17%); the high content ofkaurane, cedrane, phyllocladane, and abietane- type compounds indicate that ambers originated from conifers and are dominated by the Cupressaceae family. The δ13 C values for ambers from the Shenbei coalfield are 22. 8‰~21. 2‰, similar to those of ambers from other regions of the same period; the hydrogen isotopes are less variable (297. 2‰~276. 0‰) and the oxygen isotope values range from 17. 4‰ to 31. 6‰. The stable isotopic composition of amber (carbon, hydrogen, and oxygen) provides further evidence that amber is of gymnosperm origin. The δ13C values of amber are mainly related to global temperature variations and atmospheric composition at the time during its formation. The hydrogen and oxygen isotopic compositions of ambers from the Shenbei coalfield are mainly related to the plant species (Pinus) and the local temperature and precipitation conditions in which the plants grew. Stable carbon isotopes of ambers are expected to be a good indicator for the study of palaeoclimate and palaeo- atmospheric composition, and the hydrogen and oxygen isotopes of ambers may reflect paleoenvironmental conditions at the time of formation, but further research is needed on the stable isotope of ambers.